Bright-field analysis of phi29 DNA packaging motor using a magnetomechanical system.
نویسندگان
چکیده
We report a simple and robust magnetomechanical system for direct visual observation of the DNA packaging behavior of the bacteriophage phi29 in real time. The system comprises a micron-sized magnetic bead attached to the free end of the viral DNA, a magnet and a bright-field microscope. We show that the phi29 DNA packaging activity can be observed and dynamically analyzed at the single molecular level in bright field with a relatively simple system. With this system we also visually demonstrate the phi29 motor transporting a cargo 10 000 times the viral size.
منابع مشابه
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عنوان ژورنال:
- Applied physics letters
دوره 93 15 شماره
صفحات -
تاریخ انتشار 2008